Riffyn's Plate tab interface can be used to perform bulk transfers of resources in a step. The Plate tab allows you to specific the source plate, destination plate, and the transfer type for each well plate on your experiment. Doing so allows Riffyn to automate the entry of data for each resource (and the plates that contain them) on the step.
Using the Plate tab consists of the following steps:
- Access to the plate interface.
- Source and destination plate settings.
- Source plate mapping parameters.
- Destination plate mapping parameters.
- Adding transfer rules.
- Troubleshooting errors.
Access to the Plate Tab Interface
The Plate tab can be accessed by selecting "Plate" at the top of the bottom panel in Measure Mode. New transfer rules are added via clicking the "+" icon at the bottom left corner.
Configure source plate search parameters and destination plate settings
The two drop-down menus at the top of the panel select the types of resources to be transferred on the step. In the example below, resources from plates of type "cell culture" will be transferred to create destination plate resources of type "New cell culture".
Both source and destination well resources must contain a way to identify their location on a plate. Each resource requires a plate, row, and column identifier. By default the Plate tab interface tries to use system-defined fixed properties called "Plate ID", "Row", and "Column" to find source plate well resources, and write information to destination plate resources. It is possible to use properties other than system properties for both finding source plate resources and writing destination plate resources by selecting the configuration icons at the right of each dropdown. Details on other ways to use the Plate tab.
Source plate search parameters can be defined to help Riffyn search the inventory for the correct resources with selected property names.
Destination plate mapping parameters can be defined to allow the Plate tab interface to create output resources with properties describing the location of each well on the current step.
Add transfer rules
A new transfer rule can be added by clicking the "+" button at the bottom of the screen, next to the words "PLATE ACTIONS".
Input source plate information is entered on the left and output plate information is entered on the right. For each transfer rule users must specify
- Source plate ID.
- Source plate size.
- Transfer type (see here for a description of transfer types).
- Destination plate ID.
- Destination size.
By default, transfers are assumed to be of the "Copy" type and both source and destination plates are assumed to be of the 96-well size (8 rows x 12 columns). Additional plate sizes and transfer types (including a "no transfer" option for pass-through resource types) are available from their respective drop-down menus. For each transfer type a specific well-to-well transfer example is provided in parentheses for transfers between 96-well plates or, for Quadrant transfers, between 96- and 384-well plates. If either the transfer type or plate size needed for your experiments is missing from these drop-down menus, please let us know with an e-mail to firstname.lastname@example.org.
It is possible to copy and paste plate multiple source and destination plate IDs to and from the clipboard by first selecting multiple transfer rows (shift+click, or cmd+click) and placing focus on within the source or destination plate ID columns. Data will only be copied to/from the selected rows. IDs can also be filled down using the fill down icon in the bottom toolbar.
The rules you have selected are automatically evaluated and errors are reported on the far left side of each transfer. The Plate tab interface will report how many source resources were found in the inventory corresponding to the specified source Plate IDs. Use of the Verify button at the bottom corner rechecks all existing rules but is not necessary.
A green check mark will be displayed next to each row where:
- The number of source resources found in inventory is less than or equal to the number of wells expected from the specified format
- Each source resource is associated with a unique combination of "Row" and "Column" values
- The specified transfer type is allowed for the specified source and destination plate Sizes.
An error icon will be displayed and the "Apply" button will remain inactive if any of these conditions are not met. Clicking on the error error icon will provide feedback to help clear the error state. If no errors exist the "Apply" button can be used to trigger the plate transfers. Runs and resources will be visible in the "Actual" tab.