Creating run connections is an important feature of Riffyn. When runs are connected across steps, the data across these steps will be automatically joined together on data export, allowing a comprehensive cause/effect analysis to be performed across an entire process.
A run can be created on a step by propagation of existing runs of an upstream step to a downstream step. When runs are propagated from one step to another they are automatically connected across these steps. If runs are created manually or via file upload they will not be automatically connected across steps.
A user can connect runs in two ways:
- Run Propagation. This is when runs are added to one step and then propagated upstream or downstream to different steps. When runs are propagated across steps, they are automatically connected across the chosen Run Path.
- By connecting runs directly. Sometimes, rather than propagating runs from one step to the next, the user will want to create runs on two steps separately, and subsequently connect these runs.
There could be many reasons why it is necessary to connect runs after they have been added to steps (as opposed to adding runs to one step and propagating these runs upstream or downstream). For example, when performing an experiment on the HPLC of Cell Culture Supernatant Public Process, it might be desirable to decide which batch(es) of Mobile Phase to use for HPLC samples at time of execution. Since runs are typically created while planning an experiment, runs on step "Prep Mobile Phases" (Prep Mobile Phases) and step "Inject Samples" (Inject Samples) can be created separately, and then connected at the time of experiment execution, by connecting runs on step "Inject Samples" to the appropriate runs on step "Prep Mobile Phases".
To demonstrate how to connect existing runs across steps, we have created Experiment E7 in the Public Process HPLC of Cell Culture Supernatant. The experiment has two runs on step "Prep Mobile Phases" and four runs on step "Inject Samples". To connect these runs follow these steps:
- Click on step "Prep Mobile Phases" in Flow Diagram. Navigate to the "1. Plan Experiment" tab. Notice that the anchor on step "Prep Mobile Phases" turns yellow.
- Click the anchor step "Inject Samples". See that the default Run Path ("Prep Mobile Phases" -> "Column Equilibration" -> "Inject Samples") gets highlighted in blue, and bottom panel changes to Flow Mode (shows runs on Steps "Prep Mobile Phases" and "Inject Samples" side-by-side).
- Select the run on step "Prep Mobile Phases" to which should be connected to runs on step "Inject Samples" (in this case, choose Run R1). Then, select the runs on step "Inject Samples" which should be connected to to runs on step"Prep Mobile Phases". (In this case choose Runs R1 and R2). Notice that the split ratio changes as more runs on step "Inject Samples". (In this example, because one run is selected on step "Prep Mobile Phases" and two runs on step "Inject Samples", the split ratio automatically changes to 1:2.). Additionally, because in this example runs are connected directly from step "Prep Mobile Phases" to step "Inject Samples", the Run Path should be changed such that "Prep Mobile Phases" is connected directly to "Inject Samples" by choosing the "blank" selection from the dropdown menu next to "Create Connected Runs from "Prep Mobile Phases" to "Inject Samples" via..." on the bottom task bar.
- Click "GO" to connect the runs. Notice that the Runs on step "Inject Samples" get highlighted in blue to indicate the connection:
- If desired, connect Run R2 on step "Prep Mobile Phases" to one or more of the unconnected runs on step "Inject Samples" using the same procedure outlined above.