Connecting Runs across Steps

Creating run connections is an important feature of Riffyn. When runs are connected across steps, the data across these steps will be automatically joined together on data export, allowing you to determine the relationships of each material across an entire process. 

A run can be created on a step by propagation of existing runs of an upstream step to a downstream stepWhen runs are propagated from one step to another they are automatically connected across these steps. If runs are created manually or via file upload they will not be automatically connected across steps. 

In the HPLC of Cell Culture Supernatant public process, connecting runs enables assessment of the impact of the centrifugation temperature used to generate cell culture supernatant (data generated on "Centrifuge Samples" step) on the concentration of acetate found in this supernatant (data generated on "Inject Samples" step). If runs are created for these two steps but not connected, then the data across these steps will not be joined, and this cause/effect analysis will not be possible.  

To demonstrate how to propagate runs, use the Public Process HPLC of Cell Culture Supernatant. In this demo, step "Prep Mobile Phases" has one run (named "Mobile Phase A"). All of the following examples show the propagation of a run from an upstream step to downstream step(s), but it is important to note that propagation from a downstream step to an upstream step is also possible.

  1. Propagating a run from one step to the next with a 1:1 split

  2. Propagating a run from one step to the next with a 1:N split

  3. Propagating a run from one step to multiple steps with a 1:1 split

  4. Propagating a run from one step through multiple steps with a 1:N split

  5. Changing the Run Path when propagating runs

Each of the below method can also be done by selecting a run aggregation instead of a singular run. More details on run aggregations.

Propagating a run from one step to the next with a 1:1 split

A connection from a run in one step to a run in another can be achieved with a "1:1 split", meaning each run on the first step is connected to one and only one run on the next step.

For example, a batch of runs could be created for the "Prep Mobile Phase" step, each of which should have a single run in the "Column Equilibration" step. In that case, runs should be propagated from the "Prep Mobile Phases" step to the "Column Equilibration" step:

  1. In the Flow Diagram, click on a step with run(s) on it; in this example, "Prep Mobile Phases" has one run on it. Notice that a yellow anchor symbol appears in the lower left corner of the "Prep Mobile Phases" step. 
  2. Click the anchor on the step to which you'd like to propagate the runs (in this example, "Column Equilibration").
    Screen_Shot_2018-08-27_at_4.17.10_PM.png
  3. The connected steps turn blue in the Flow Diagram, and the Bottom Panel switches to "Flow Mode" (showing two steps side by side).
    Screen_Shot_2018-08-27_at_4.45.27_PM.png
  4. Individual runs can be chosen by clicking the box next to the run, or all runs can be selected by checking the box to the left of the "Label" Header.
  5. Choose the split ratio after you have selected runs. The split ratio indicates how many runs to create in the connected step. The default is 1:1 (meaning one run will be created in the connected step for every one run in the original step). In this example we will use a split ratio of 1:1. For different split ratios, see Example 2.
    Screen_Shot_2018-08-31_at_1.08.27_PM.png
    Screen_Shot_2018-08-31_at_1.06.39_PM.pngClick the "Go" button to make a connected run on "Column Equilibration".
  6. To view the run connection, select a run by checking the box next to a run. In this example, the run on "Prep Mobile Phases" is selected. Notice that the run on step "Column Equilibration" is highlighted in blue, indicating that these runs are connected.
  7. Notice that the Mobile Phase resource output in "Column Equilibration" step (GR-XXXX), has been assigned as the Mobile Phase input of the run on "Column Equilibration". This connection of runs allows the resources used across the runs to be automatically connected.

Propagating a run from one step to the next with a 1:N split

A connection from a run in one step to many runs in another can be achieved with a "1:N split", meaning each run on the first step is connected to more than one runs on the next step. 

For example, a batch of runs could be created for the "Prep Mobile Phase" step, each of which should have a multiple runs in the "Column Equilibration" step. A 1:3 split of runs can be propagated from the "Prep Mobile Phases" step to the "Column Equilibration" step as follows:

  1. Similar to Example 1 you can select the split ratio when selecting runs in the flow interface. In this case you can list a split ratio of 1:3.
    Screen_Shot_2018-08-31_at_1.09.34_PM.png
  2. After clicking "GO", "Column Equilibration" will have 3 runs placed on it, each of which is connected to the run on "Prep Mobile Phases" (as evidenced by the blue highlighting that appears on the "Column Equilibration" runs when the run on "Prep Mobile Phases" selected).
    Screen_Shot_2018-08-28_at_8.20.21_AM.png

Propagating a run from one step to multiple steps with a 1:1 split

A connection from a run in one step to a run in multiple other steps can be achieved with a "1:1 split", meaning each run on the first step is connected to one and only one run on multiple steps.

For example, a batch of runs could be created for the "Prep Mobile Phase" step, each of which should have a single run in the "Column Equilibration" step. In that case, runs should be propagated from the "Prep Mobile Phases" step to the "Column Equilibration" step:

A batch of runs could be created for the "Prep Mobile Phase" step, each of which would have a single run in the "Column Equilibration" step. A sample is then injected in the "Inject Sample" step after "Column Equilibration". Therefore a run on the "Prep Mobile Phases" step needs to be connected to a run on the "Column Equilibration" step, and subsequently each run in the "Column Equilibration" step should be be connected to a run on the "Inject Samples" step. Rather than propagating these runs by performing 2 propagations, you can create connected runs on both the "Column Equilibration" and "Inject Sample" steps at the same time, by propagating runs from "Prep Mobile Phases" to "Inject Sample" through "Column Equilibration":

  1. Similar to the first examples you can click the anchor to enter the flow interface. The design canvas will show connected steps along the default "Run Path" turn blue. Optional paths will remain grey.
    Screen_Shot_2018-08-28_at_8.24.57_AM.png
  2. Similar to the past examples, the flow interface gives the option to select the split ratio once you have selected runs. In this case and additional option "...via" is available. This allows you to select which path you want runs to move through. Notice that changing this option also changes the "Default" blue highlighting in the design canvas.
    Screen_Shot_2018-08-31_at_1.15.17_PM.png
  3. The "Split after" option allows you to specify when you want runs to split. See Example 4 for more details.
  4. Click the "Go" button to create and connect the runs. This will connect runs at the listed split ration, via the selected step. Similar to the previous examples you can use the flow interface to view connections.

Propagating a run from one step through multiple steps with a 1:N split

A connection from a run in one step to many runs in multiple steps can be achieved with a "1:N split", meaning each run on the first step is connected to more than one run on the other steps. 

A batch of runs could be created for the "Prep Mobile Phase" step, each of which could have a single run in the "Column Equilibration" step. Three samples are then injected in the "Inject Sample" step after "Column Equilibration". Therefore a run on the "Prep Mobile Phases" step needs to be connected to a run on the "Column Equilibration" step, and subsequently each run in the "Column Equilibration" step should be be connected to three runs on the "Inject Samples" step:

  1. Following the same process as previous examples you can set the split ratio across the "Column Equilibration" and "Inject Samples" step.
  2. Because a 1:1 ratio is needed from step "Prep Mobile Phases" to step "Column Equilibration" (one batch of Mobile Phase is connected with one "Column Equilibration" run), a 1:3 ratio can be set to occur after "Column Equilibration" step. 
    Screen_Shot_2018-08-28_at_8.37.33_AM.png
  3. In this case 3 runs are created on step "Inject Samples", which are connected to step "Column Equilibration", as shown by the blue highlighting that appears on these runs when the run on step "Prep Mobile Phases" is selected. The runs on step "Inject Samples" are therefore connected to step "Prep Mobile Phases" via step "Column Equilibration" as shown by blue highlighting and directional arrows on the Process Flow Diagram.
    Screen_Shot_2018-08-28_at_8.39.29_AM.png

    However, only one run is created on step "Column Equilibration". This one run on step "Column Equilibration" is connected to one run on step "Prep Mobile Phases", but to three runs on step "Inject Samples".
    Screen_Shot_2018-08-28_at_8.39.57_AM.png

Changing the Run Path when propagating runs

When an output of a step is connected to more than one downstream step, a choice exists as to which run path to use when propagating runs. In the Public Process "HPLC of Cell Culture Supernatants", step "Prep Mobile Phases" has a single output, "Mobile Phase", which is connected to both step "Column Equilibration" and step "Inject Samples". When you propagate runs from "Prep Mobile Phases" to "Inject Samples", you can either propagate runs through "Column Equilibration" (meaning, runs on "Inject Samples" will be connected to runs on "Prep Mobile Phases" via runs on "Column Equilibration") or you can propagate runs directly from "Prep Mobile Phases" to "Inject Samples" and bypass "Column Equilibration". Example 3 demonstrated how to propagate runs from "Prep Mobile Phases" to "Inject Samples" via "Column Equilibration". This example demonstrates how to propagate runs from "Prep Mobile Phases" directly to "Inject Samples", skipping "Column Equilibration". Two use cases exist; if a batch of Mobile Phase skips the "Column Equilibration" step and goes straight to injecting samples; or if one batch of Mobile Phase is used for "Column Equilibration" and another batch is used for sample injection. 

  1. Similar to past examples you can select a step and enter the flow interface using the anchor. You can select an alternative the run path by choosing the "–" selection from the dropdown menu next to "Create Connected Runs from "Prep Mobile Phases" to  "Inject Samples" via..."
    Screen_Shot_2018-08-28_at_9.00.27_AM.png
  2. Notice that the run path changes in the Process Flow Diagram. "Column Equilibration" turns gray, while Steps "Prep Mobile Phases" and "Inject Samples" remain blue, indicating that newly propagated runs from "Prep Mobile Phases" will bypass step "Column Equilibration" and flow directly to "Inject Samples".
    Screen_Shot_2018-08-28_at_9.01.06_AM.png

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